Efficacy of BACTI-NIL®AQUA in Experimental infection with Vibrio parahaemolyticus in juvenile of Litopenaeus vannamei

Abstract

This work aims to evaluate looks at the antibacterial efficacy of BACTI- NIL®AQUA when added to the feed of Penaeus vannamei in experimental infection with Vibrio parahaemolyticus (M0904AHPND+strain). Results show that adition of BACTI-NIL®AQUA at 3,000 and 5,000 part per million (ppm), causes inhibition growth zones of 15.00 ± 0.50 milimeters (mm) and 17.00 ± 0.30 mm, respectively. The challenge with V. parahaemolyticus resulted in 60% survival for organisms fed doses 3,000 ppm of BACTI-NIL®AQUA and 60% for those fed 5,000 ppm of BACTI-NIL®AQUA, resulting in twice the amount of survival as opposed to 13.33% (4 organism) in the positive control at 24 hours post-infection. Histopathological alterations in the hepatopancreas with hemocytic infiltration within the intertubular connective tissue were observed. Also, tubules with severe cell detachment and tubular atrophy were detected in the positive control organisms, and organisms treated with of BACTI-NIL®AQUA only had vermiform structures in the tubular lumen, cell detachment and infiltration of hemolymph in intertubular connective tissue. According to the analysis of the studied variables, it can be concluded that of BACTI-NIL®AQUA is a promising alternative for V. parahaemolyticus control in shrimp culture.